Eniyan CYP2C19 Gene Polymorphism

Apejuwe kukuru:

Ohun elo yii ni a lo fun wiwa agbara in vitro ti polymorphism ti awọn Jiini CYP2C19 CYP2C19 * 2 (rs4244285, c.681G>A), CYP2C19 * 3 (rs4986893, c.636G>A), CYP2C12, 240.6 (rs4244285) T) ni DNA jinomiki ti gbogbo awọn ayẹwo ẹjẹ eniyan.


Alaye ọja

ọja Tags

Orukọ ọja

HWTS-GE012A-Eniyan CYP2C19 Gene Polymorphism Apo Awari (Pluorescence PCR)

Iwe-ẹri

CE

Arun-arun

CYP2C19 jẹ ọkan ninu awọn enzymu metabolizing oogun pataki ninu idile CYP450.Ọpọlọpọ awọn sobusitireti endogenous ati nipa 2% ti awọn oogun ile-iwosan jẹ iṣelọpọ nipasẹ CYP2C19, gẹgẹbi iṣelọpọ ti awọn inhibitors aggregation antiplatelet (bii clopidogrel), awọn inhibitors pump proton (omeprazole), anticonvulsants, bbl CYP2C19 pupọ polymorphisms tun ni awọn iyatọ ninu agbara iṣelọpọ ti iṣelọpọ agbara ti o ni ibatan oloro.Awọn iyipada aaye wọnyi ti * 2 (rs4244285) ati * 3 (rs4986893) fa isonu ti iṣẹ ṣiṣe enzymu ti a fiwe si nipasẹ jiini CYP2C19 ati ailagbara ti agbara sobusitireti ti iṣelọpọ, ati mu ifọkansi ẹjẹ pọ si, nitorinaa lati fa awọn aati oogun ti ko dara ti o ni ibatan si ifọkansi ẹjẹ.* 17 (rs12248560) le ṣe alekun iṣẹ ṣiṣe henensiamu ti koodu nipasẹ jiini CYP2C19, iṣelọpọ ti awọn metabolites ti nṣiṣe lọwọ, ati mu idinamọ akojọpọ platelet pọ si ati mu eewu ẹjẹ pọ si.Fun awọn eniyan ti o ni iṣelọpọ ti o lọra ti awọn oogun, gbigbe awọn iwọn deede fun igba pipẹ yoo fa majele to ṣe pataki ati awọn ipa ẹgbẹ: nipataki ibajẹ ẹdọ, ibajẹ eto hematopoietic, ibajẹ eto aifọkanbalẹ aarin, ati bẹbẹ lọ, ti o le ja si iku ni awọn ọran ti o lagbara.Gẹgẹbi awọn iyatọ ti ara ẹni kọọkan ninu iṣelọpọ oogun ti o baamu, o pin ni gbogbogbo si awọn ẹya phenotypes mẹrin, eyun iṣelọpọ iyara-yara (UM, * 17/*17, * 1/*17), iṣelọpọ iyara (RM, * 1/*1) ) , iṣelọpọ agbedemeji (IM, * 1/*2, * 1/*3), iṣelọpọ ti o lọra (PM, * 2/*2, * 2/* 3, * 3/*3).

ikanni

FAM CYP2C19*2
CY5 CYP2C9*3
ROX CYP2C19*17
VIC/HEX IC

Imọ paramita

Ibi ipamọ Omi: ≤-18℃
Selifu-aye 12 osu
Apeere Iru Ẹjẹ anticoagulated EDTA tuntun
CV ≤5.0%
LoD 1.0ng/μL
Ni pato Ko si ifasilẹ-agbelebu pẹlu awọn ilana deede ti o ga pupọ (jiini CYP2C9) ninu jiini eniyan.Awọn iyipada ti CYP2C19 * 23, CYP2C19 * 24 ati awọn aaye CYP2C19 * 25 ni ita ibiti a ti rii ti ohun elo yii ko ni ipa lori ipa wiwa ohun elo yii.
Awọn ohun elo ti o wulo Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Yara Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR eto

LineGene 9600 Plus Real-Time PCR erin System

MA-6000 Real-Time Quantitative Gbona Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Sisan iṣẹ

Reagenti isediwon ti a ṣe iṣeduro:Makiro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ati Makiro & Micro-Test Laifọwọyi Nucleic Acid Extractor (HWTS-3006).


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