Kokoro Zika
Orukọ ọja
HWTS-FE002 Apo Iwari Acid Nucleic Acid (Fluorescence PCR) Zika Virus
Iwe-ẹri
CE
Arun-arun
Kokoro Zika jẹ ti iwin Flaviviridae, jẹ ọlọjẹ RNA rere ti o ni okun-ọkan kan pẹlu iwọn ila opin ti 40-70nm.O ni apoowe kan, o ni awọn nucleotides 10794, ati koodu 3419 amino acids.Gẹgẹbi genotype, o pin si iru Afirika ati iru Asia.Arun kokoro Zika jẹ arun ajakalẹ-arun nla ti o ni opin ti ara ẹni ti o fa nipasẹ ọlọjẹ Zika, eyiti o tan kaakiri nipasẹ jijẹ ti awọn ẹfọn Aedes aegypti.Awọn ẹya ara ile-iwosan jẹ nipataki iba, sisu, arthralgia tabi conjunctivitis, ati pe o ṣọwọn apaniyan.Gẹgẹbi Ajo Agbaye ti Ilera, microcephaly ọmọ tuntun ati iṣọn Guillain-Barre (aisan Guillain-Barré) le ni nkan ṣe pẹlu akoran ọlọjẹ Zika.
ikanni
| FAM | Zika kokoro nucleic acid |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤30℃ & aabo lati ina |
| Selifu-aye | 12 osu |
| Apeere Iru | omi ara tuntun |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500ng/μL |
| Ni pato | Awọn abajade idanwo ti a gba nipasẹ ohun elo yii kii yoo ni ipa nipasẹ haemoglobin (<800g/L), bilirubin (<700μmol/L), ati awọn lipids / triglycerides (<7mmol/L) ninu ẹjẹ. |
| Awọn ohun elo ti o wulo | ABI 7500 Real-Time PCR Systems ABI 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Eto PCR akoko-gidi, BioRad CFX Opus 96 Eto PCR akoko-gidi |
Sisan iṣẹ
Aṣayan 1.
QIAamp Viral RNA Mini Kit(52904), Iyọkuro Acid Nucleic tabi Reagent Iwẹnumọ (YDP315-R) nipasẹ Tiangen Biotech (Beijing) Co., Ltd.Awọn isediwonyẹ ki o fa jade ni ibamu si awọn ilana isediwon, ati iwọn didun isediwon ti a ṣe iṣeduro jẹ 140 μL ati iwọn didun elution ti a ṣe iṣeduro jẹ 60 μL.
Aṣayan 2.
Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ati Makiro & Micro-Test Laifọwọyi Nucleic Acid Extractor (HWTS-3006).Awọn isediwon yẹ ki o wa jade ni ibamu si awọn ilana.Iwọn ayẹwo isediwon jẹ 200 μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
