Enterococcus-sooro Vancomycin ati Gene-sooro Oògùn
Orukọ ọja
HWTS-OT090-Vancomycin-sooro Enterococcus ati Apo Iwari Jiini ti ko ni oogun (Pluorescence PCR)
Arun-arun
Resistance si Oògùn ni a tun mọ bi resistance oogun, tọka si resistance ti awọn kokoro arun si iṣe ti awọn oogun antibacterial.Ni kete ti resistance oogun ba waye, ipa chemotherapy ti awọn oogun yoo dinku ni pataki.Resistance si Oògùn ti pin si atorunwa resistance ati ipasẹ resistance.Idaabobo inu inu jẹ ipinnu nipasẹ awọn jiini chromosomal ti kokoro arun, ti o kọja lati irandiran, ati pe kii yoo yipada.Idaduro ti o gba jẹ nitori otitọ pe lẹhin olubasọrọ pẹlu awọn egboogi, awọn kokoro arun yi awọn ipa ọna ti iṣelọpọ ti ara wọn pada ki wọn ko ba pa nipasẹ awọn egboogi.
Awọn jiini resistance vancomycin VanA ati VanB jẹ ipasẹ resistance oogun, laarin eyiti VanA ṣe afihan awọn ipele giga ti resistance si vancomycin ati teicoplanin, VanB ṣe afihan awọn ipele oriṣiriṣi ti resistance si vancomycin, ati pe o ni itara si teicoplanin.A maa n lo Vancomycin ni ile-iwosan lati tọju awọn akoran kokoro-arun Giramu-positive, ṣugbọn nitori ifarahan ti vancomycin-sooro enterococci (VRE), paapaa enterococcus faecalis ati enterococcus faecium, ṣiṣe iṣiro diẹ sii ju 90%, o ti mu awọn italaya nla tuntun wa si itọju ile-iwosan. .Lọwọlọwọ, ko si oogun antibacterial kan pato fun itọju VRE.Kini diẹ sii, VRE tun le ṣe atagba awọn jiini resistance oogun si awọn enterococci miiran tabi awọn kokoro arun Giramu miiran.
ikanni
| FAM | Vancomycin-sooro enterococci (VRE): Enterococcus faecalis ati Enterococcus faecium |
| VIC/HEX | Iṣakoso ti abẹnu |
| CY5 | vancomycin resistance pupọ VanB |
| ROX | vancomycin resistance pupọ VanA |
Imọ paramita
| Ibi ipamọ | Omi: ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | sputum, ẹjẹ, ito tabi awọn ileto mimọ |
| CV | ≤5.0% |
| Ct | ≤36 |
| LoD | 103CFU/ml |
| Ni pato | Ko si ifaseyin agbelebu pẹlu awọn aarun atẹgun miiran bii klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, streptococcus pneumoniae, neisseria meningitidis, staphylococcus aureus, klebsiella oxytoca, haemophilus. escherichia coli, pseudomonas fluorescens, candida albicans, chlamydia pneumoniae, atẹgun adenovirus, tabi awọn ayẹwo ni awọn Jiini-sooro oogun miiran CTX, mecA, SME, Awọn ayẹwo SHV ati TEM. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR System SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto |
Sisan iṣẹ
Niyanju isediwon reagents: Makiro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) ati Makiro & Micro-Idanwo Aifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) .
