Staphylococcus Aureus ati Meticillin-Atako Staphylococcus Aureus Nucleic Acid
Orukọ ọja
HWTS-OT062-Staphylococcus Aureus ati Methicillin-Resistant Staphylococcus Aureus Nucleic Acid Apo (Fluorescence PCR)
Iwe-ẹri
CE
Arun-arun
Staphylococcus aureus jẹ ọkan ninu awọn kokoro arun pathogenic pataki ti ikolu nosocomial.Staphylococcus aureus (SA) jẹ ti staphylococcus ati pe o jẹ aṣoju ti awọn kokoro arun Gram-positive, eyiti o le ṣe awọn orisirisi awọn majele ati awọn enzymu apanirun.Awọn kokoro arun ni awọn abuda ti pinpin jakejado, pathogenicity ti o lagbara ati oṣuwọn resistance giga.Jiini nuclease thermostable (nuc) jẹ jiini ti o tọju pupọ ti staphylococcus aureus.Ni awọn ọdun aipẹ, nitori lilo lọpọlọpọ ti awọn homonu ati awọn igbaradi ajẹsara ati ilokulo ti awọn oogun apakokoro gbooro, awọn akoran ile-iṣẹ ti o fa nipasẹ Staphylococcus aureus-sooro Methicillin (MRSA) ni Staphylococcus ti wa ni igbega.Oṣuwọn wiwa apapọ orilẹ-ede ti MRSA jẹ 30.2% ni ọdun 2019 ni Ilu China.MRSA ti pin si MRSA ti o ni ibatan ilera (HA-MRSA), MRSA ti agbegbe (CA-MRSA), ati MRSA ti o ni ibatan ẹran-ọsin (LA-MRSA).CA-MRSA, HA-MRSA, LA-MRSA ni awọn iyatọ nla ni microbiology, resistance bacterial (fun apẹẹrẹ, HA-MRSA fihan diẹ sii resistance ti awọn oògùn ju CA-MRSA) ati awọn abuda iwosan (fun apẹẹrẹ aaye ikolu).Gẹgẹbi awọn abuda wọnyi, CA-MRSA ati HA-MRSA le ṣe iyatọ.Sibẹsibẹ, awọn iyatọ laarin CA-MRSA ati HA-MRSA n dinku nitori iṣipopada igbagbogbo ti awọn eniyan laarin awọn ile-iwosan ati agbegbe.MRSA jẹ sooro oogun-ọpọlọpọ, kii ṣe sooro si awọn egboogi β-lactam nikan, ṣugbọn si aminoglycosides, macrolides, tetracyclines ati quinolones si awọn iwọn oriṣiriṣi.Awọn iyatọ agbegbe nla wa ni awọn oṣuwọn resistance oogun ati awọn aṣa oriṣiriṣi.
Jiini meticillin resistance mecA ṣe ipa ipinnu kan ninu resistance staphylococcal.Jiini naa ni a gbe sori ẹya jiini alagbeka alailẹgbẹ kan (SCCmec), eyiti o ṣe koodu amuaradagba-abuda penicillin 2a (PBP2a) ati pe o ni ibatan kekere si awọn egboogi β-lactam, nitorinaa awọn oogun antimicrobial ko le ṣe idiwọ iṣelọpọ ti Layer peptidoglycan ogiri sẹẹli, Abajade ni oògùn resistance.
ikanni
| FAM | Jiini mecA-sooro meticillin |
| CY5 | staphylococcus aureus nuc jiini |
| VIC/HEX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | Omi: ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | sputum, awọ ara ati awọn ayẹwo àkóràn àsopọ rirọ, ati gbogbo awọn ayẹwo ẹjẹ |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/ml |
| Ni pato | Ko si ifaseyin agbelebu pẹlu awọn aarun atẹgun miiran bii methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, staphylococcus epidermidis-sooro methicillin, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniater baacabiliate, klebsiella pneumoniate, acinumoniae, klebsiella pneumoniate, acinumoniae, acinumoniae, klebsiella pneumoniae, acinumoniae, acinumoniae, acinumoniae. streptococcus pneumonia , enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
