Mycobacterium Tuberculosis INH Resistance
Orukọ ọja
HWTS-RT002A-Mycobacterium Tuberculosis Isoniazid Resistance Detection Kit (Pluorescence PCR)
Arun-arun
Isoniazid, oogun pataki kan ti o lodi si ikọ-igbẹ ti a ṣe ni 1952, jẹ ọkan ninu awọn oogun ti o munadoko julọ fun itọju apapọ ti iko-ara ti nṣiṣe lọwọ ati oogun kan fun iko-ara ti o wa latent.
KatG jẹ jiini akọkọ fifi koodu catalase-peroxidase ati iyipada jiini katG le ṣe igbelaruge iṣelọpọ ti ogiri sẹẹli mycolic acid, ṣiṣe awọn kokoro arun sooro si isoniazid.Ọrọ ikosile ti KatG jẹ ni ibamu pẹlu odi pẹlu awọn ayipada ninu INH-MIC, ati idinku 2-agbo ni awọn abajade ikosile katG ni ilosoke 2-agbo diẹ diẹ ninu MIC.Idi miiran ti isoniazid resistance ni mycobacterium tuberculosis waye nigbati ifibọ ipilẹ, piparẹ tabi iyipada ba waye ninu ibi Jiini InhA ti iko mycobacterium.
ikanni
| ROX | inhA (-15C>T) ojula · |
| CY5 | katG (315G>C) ojula |
| VIC (HEX) | IS6110 |
Imọ paramita
| Ibi ipamọ | ≤-18℃ Ninu okunkun |
| Selifu-aye | 12 osu |
| Apeere Iru | Tutu |
| CV | ≤5.0% |
| LoD | 1 × 103kokoro arun/ml |
| Ni pato | Aisi-agbelebu ifaseyin pẹlu awọn iyipada ti awọn aaye resistance oogun mẹrin (511, 516, 526 ati 531) ti jiini rpoB ni ita ibiti wiwa ti ohun elo wiwa. Awọn irinṣẹ to wulo: Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
