Papillomavirus eniyan (Awọn oriṣi 28) Apo Iwari Genotyping (Pluorescence PCR)
Orukọ ọja
HWTS-CC004A-Papillomavirus eniyan (Awọn oriṣi 28) Ohun elo Iwari Jiini (Pluorescence PCR)
Arun-arun
Awọn ohun elo naa nlo ọpọ nucleic acid ampilifaya (PCR) ọna iwari fluorescence.Awọn alakoko pato ati awọn iwadii jẹ apẹrẹ ti o da lori ọkọọkan ibi-afẹde pupọ L1 ti HPV.Iwadii kan pato jẹ aami pẹlu FAM fluorophore (HPV6, 16, 26, 40, 53, 58, 73), VIC/HEX fluorophore (HPV11, 18, 33, 43, 51, 59, 81), CY5 fluorophore (HPV35, 4). , 45, 54, 56, 68, 82) ati ROX fluorophore (HPV31, 39, 42, 52, 61, 66, 83) ni 5', ati ẹgbẹ 3' quencher jẹ BHQ1 tabi BHQ2.Lakoko imudara PCR, awọn alakoko kan pato ati awọn iwadii so mọ awọn ilana ibi-afẹde kọọkan wọn.Nigbati Taq henensiamu ba pade awọn iwadii ti a dè si ọkọọkan ibi-afẹde, o ṣiṣẹ iṣẹ ti 5 'opin exonuclease lati yapa fluorophore onirohin kuro ninu fluorophore quencher, ki eto ibojuwo fluorescence le gba ifihan fluorescent, iyẹn ni, ni gbogbo igba ti DNA kan. okun ti pọ si, a ti ṣẹda moleku Fuluorisenti kan, eyiti o mọ imuṣiṣẹpọ pipe ti ikojọpọ ti awọn ifihan agbara Fuluorisenti ati dida awọn ọja PCR, lati ṣaṣeyọri didara ati wiwa genotyping ti awọn acids nucleic ti awọn oriṣi 28 ti papillomavirus eniyan ni awọn ayẹwo sẹẹli exfoliated cervical. .
ikanni
| FAM | 16,58,53,73,6,26,40· |
| VIC/HEX | 18,33,51,59,11,81,43 |
| ROX | 31,66,52,39,83,61,42 |
| CY5 | 56,35,45,68,54,44,82 |
Imọ paramita
| Ibi ipamọ | ≤-18℃ Ninu okunkun |
| Selifu-aye | 12 osu |
| Apeere Iru | Awọn sẹẹli exfoliated cervical |
| Ct | ≤25 |
| CV | ≤5.0% |
| LoD | 25 idaako / lenu |
| Awọn ohun elo ti o wulo | Rọrun Amp Gidigidi Fluorescence Eto Wiwa Isothermal (HWTS1600)
Applied Biosystems 7500 Real-Time PCR Systems
Applied Biosystems 7500 Yara Real-Time PCR Systems
QuantStudio®5 Real-Time PCR Systems
SLAN-96P Real-Time PCR Systems
LightCycler®480 Real-Time PCR eto
LineGene 9600 Plus Real-Time PCR erin System
MA-6000 Real-Time Quantitative Gbona Cycler
BioRad CFX96 Real-Time PCR System
BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Aṣayan 1.
Niyanju isediwon reagent: Makiro & Micro-Test Ayẹwo Tu Reagent (HWTS-3005-8).
Aṣayan 2.
Niyanju isediwon reagent: Makiro & Micro-Igbeyewo Gbogun ti DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ati Makiro & Micro-Idanwo Aifọwọyi Nucleic Acid Extractor (HWTS-3006).
