Eniyan CYP2C9 ati VKORC1 Gene Polymorphism
Orukọ ọja
HWTS-GE014A-Eniyan CYP2C9 ati VKORC1 Gene Polymorphism Apo Awari (Fluorescence PCR)
Iwe-ẹri
CE
Arun-arun
Warfarin jẹ oogun apakokoro ti ẹnu ti o wọpọ ti a lo ni adaṣe ile-iwosan lọwọlọwọ, eyiti a pinnu ni pataki fun idena ati itọju awọn arun thromboembolic.Sibẹsibẹ, warfarin ni ferese itọju ti o lopin ati pe o yatọ pupọ laarin awọn ẹya ati awọn eniyan kọọkan.Awọn iṣiro ti fihan pe iyatọ ti iwọn iduroṣinṣin ni awọn eniyan oriṣiriṣi le jẹ diẹ sii ju awọn akoko 20 lọ.Ẹjẹ ifa ikọlu waye ni 15.2% ti awọn alaisan ti o mu warfarin ni ọdun kọọkan, eyiti 3.5% ti dagbasoke ẹjẹ iku.Awọn ijinlẹ elegbogi ti fihan pe polymorphism jiini ti enzymu afojusun VKORC1 ati enzyme ti iṣelọpọ CYP2C9 ti warfarin jẹ ifosiwewe pataki ti o ni ipa lori iyatọ ninu iwọn lilo warfarin.Warfarin jẹ onidalẹkun kan pato ti Vitamin K epoxide reductase (VKORC1), ati nitorinaa ṣe idiwọ iṣelọpọ ifosiwewe didi ti o kan Vitamin K ati pe o pese anticoagulation.Nọmba nla ti awọn ijinlẹ ti fihan pe pupọpupo polymorphism ti olupolowo VKORC1 jẹ ifosiwewe pataki julọ ti o kan ije ati awọn iyatọ kọọkan ni iwọn lilo ti warfarin ti a beere.Warfarin jẹ metabolized nipasẹ CYP2C9, ati awọn ẹda ara rẹ dinku iṣelọpọ ti warfarin pupọ.Awọn ẹni kọọkan ti o nlo warfarin ni ewu ti o ga julọ (lẹẹmeji si igba mẹta ti o ga julọ) ti ẹjẹ ni ipele ibẹrẹ ti lilo.
ikanni
| FAM | VKORC1 (-1639G>A) |
| CY5 | CYP2C9*3 |
| VIC/HEX | IC |
Imọ paramita
| Ibi ipamọ | Omi: ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | Ẹjẹ anticoagulated EDTA tuntun |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Ni pato | Ko si ifasilẹ-agbelebu pẹlu ọna deede ti o ga julọ ti jiini eniyan (jiini CYP2C19 eniyan, apilẹṣẹ RPN2 eniyan);iyipada ti CYP2C9*13 ati VKORC1 (3730G>A) ni ita ibiti a ti rii ti ohun elo yii |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Niyanju isediwon reagent: Makiro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ati Makiro & Micro-Idanwo Aifọwọyi Nucleic Acid Extractor(HWTS-) 3006).
