Human BCR-ABL Fusion Gene iyipada
Orukọ ọja
HWTS-GE010A-Eniyan BCR-ABL Fusion Gene Iyipada Apo Awari (Pluorescence PCR)
Arun-arun
Chronic myelogenousleukemia (CML) jẹ arun ti o buruju ti clonal ti awọn sẹẹli hematopoietic.Diẹ sii ju 95% ti awọn alaisan CML gbe chromosome Philadelphia (Ph) ninu awọn sẹẹli ẹjẹ wọn.Pathogenesis akọkọ ti CML jẹ atẹle yii: Jiini idapọ BCR-ABL jẹ ipilẹṣẹ nipasẹ gbigbe laarin abl proto-oncogene (Abelson murine leukemia viral oncogene homolog 1) lori apa gigun ti chromosome 9 (9q34) ati agbegbe iṣupọ breakpoint ( BCR) jiini lori apa gigun ti chromosome 22 (22q11);amuaradagba idapọmọra ti a fiwe si nipasẹ jiini yii ni iṣẹ ṣiṣe tyrosine kinase (TK), o si mu awọn ipa ọna ifihan agbara rẹ ṣiṣẹ (gẹgẹbi RAS, PI3K, ati JAK/STAT) lati ṣe agbega pipin sẹẹli ati ki o dẹkun apoptosis sẹẹli, ṣiṣe awọn sẹẹli pọ si ni buburu, ati nitorinaa nfa iṣẹlẹ ti CML.BCR-ABL jẹ ọkan ninu awọn itọkasi iwadii aisan pataki ti CML.Iyipada iyipada ti ipele iwe-kikọ rẹ jẹ itọkasi ti o gbẹkẹle fun idajọ asọtẹlẹ ti aisan lukimia ati pe o le ṣee lo lati ṣe asọtẹlẹ atunsan ti aisan lukimia lẹhin itọju.
ikanni
| FAM | Jiini idapọmọra BCR-ABL |
| VIC/HEX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | Omi: ≤-18℃ Ninu okunkun |
| Selifu-aye | Omi: 9 osu |
| Apeere Iru | Awọn ayẹwo ọra inu egungun |
| LoD | 1000 idaako/ml |
| Ni pato
| Ko si ifasilẹ-agbelebu pẹlu awọn Jiini idapọ miiran TEL-AML1, E2A-PBX1, MLL-AF4, AML1-ETO, ati PML-RARa |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio® 5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
