Herpes Simplex Iwoye Iru 2 Nucleic Acid
Orukọ ọja
HWTS-UR007A-Herpes Simplex Iwoye Iru 2 Ohun elo Iwari Acid Nucleic (Fluorescence PCR)
Lilo ti a pinnu
Ohun elo yii ni a lo fun wiwa didara ti ọlọjẹ Herpes simplex iru 2 nucleic acid ninu swab uretral akọ ati awọn ayẹwo swab cervical abo.
Arun-arun
Herpes Simplex Iwoye Iru 2 (HSV2) jẹ ọlọjẹ ipin kan ti a ṣepọ pẹlu tegument, capsid, mojuto, ati apoowe, ati pe o ni DNA laini ila-meji.Kokoro Herpes le wọ inu ara nipasẹ olubasọrọ taara tabi ibaraẹnisọrọ ibalopo pẹlu awọ ara ati awọn membran mucous, o si pin si akọkọ ati loorekoore.Ikolu apa ibisi jẹ eyiti o fa nipasẹ HSV2, awọn alaisan ọkunrin ni a farahan bi ọgbẹ penile, ati pe awọn alaisan obinrin farahan bi ọgbẹ, ọgbẹ, ati ọgbẹ inu.Awọn akoran akọkọ ti ọlọjẹ Herpes abe jẹ awọn akoran ipadasẹhin pupọ julọ, ayafi fun awọn herpes agbegbe diẹ pẹlu awọn membran mucous tabi awọ ara, pupọ julọ eyiti ko ni awọn ami aisan ile-iwosan ti o han gbangba.Abe Herpes ikolu ni o ni awọn abuda kan ti igbesi aye kokoro rù ati ki o rọrun ti nwaye, ati awọn mejeeji alaisan ati ẹjẹ ni o wa ni orisun ti ikolu ti arun.Ni Ilu China, oṣuwọn rere serological ti HSV2 jẹ nipa 10.80% si 23.56%.Ipele ikolu HSV2 le pin si ikolu akọkọ ati ikolu loorekoore, ati nipa 60% ti awọn alaisan ti o ni arun HSV2 tun pada.
Arun-arun
FAM: Herpes Simplex Iwoye Iru 2 (HSV2) ·
VIC (HEX): Iṣakoso inu
Eto Awọn ipo Imudara PCR
Igbesẹ | Awọn iyipo | Iwọn otutu | Aago | GbaFluorescentSignalsbi beko |
1 | 1 Yiyipo | 50℃ | 5 min | No |
2 | 1 Yiyipo | 95℃ | 10 min | No |
3 | 40 Awọn iyipo | 95℃ | iṣẹju-aaya 15 | No |
4 | 58℃ | iṣẹju-aaya 31 | Bẹẹni |
Imọ paramita
Ibi ipamọ | |
Omi | ≤-18℃ Ninu okunkun |
Selifu-aye | 12 osu |
Apeere Iru | Obinrin swab cervical, Okunrin urethral swab |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50Awọn adakọ / lenu |
Ni pato | Ko si ifaseyin agbelebu pẹlu awọn aarun STD miiran, gẹgẹbi Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium ati bẹbẹ lọ. |
Awọn ohun elo ti o wulo | O le baramu awọn ohun elo PCR Fuluorisenti akọkọ lori ọja naa. Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System. |