Coxsackie Iwoye Iru A16 Nucleic Acid

Apejuwe kukuru:

A lo ohun elo yii fun wiwa agbara in vitro ti iru ọlọjẹ Coxsackie A16 nucleic acid ninu awọn swabs ọfun eniyan.


Alaye ọja

ọja Tags

Orukọ ọja

HWTS-EV025-Coxsackie Iwoye Iru A16 Apo Iwari Acid Nucleic Acid (Amudara Isothermal Probe Enzymatic)

Iwe-ẹri

CE

Arun-arun

Ohun elo yii gba Enzymatic Probe Isothermal Amplification (EPIA), ati ṣe apẹrẹ awọn alakoko kan pato ati awọn iwadii ti o ni ipilẹ RNA (rProbe) fun agbegbe ti o ni aabo pupọ ti Cox A16, ati ṣafikun Bst henensiamu ati RNaseH henensiamu ni akoko kanna, ninu eyiti osi ati Awọn opin ọtun ti ipilẹ RNA ti rProbe jẹ aami pẹlu awọn ẹgbẹ fluorescent ati quencher, lẹsẹsẹ.Lo iṣẹ ṣiṣe DNA polymerase ati iṣẹ iṣipopada okun ti henensiamu Bst lati mu ibi-afẹde pọ si lati ṣe idanwo ni iwọn otutu igbagbogbo, enzymu RNaseH le ya awọn ipilẹ RNA lori pq arabara ibi-afẹde, ki ẹgbẹ fluorescent ati quencher ti rProbe jẹ niya nipa fluorescing.Ni afikun, ajẹkù osi ti ipilẹ rProbe RNA ti o ku le ṣee lo bi alakoko lati fa siwaju lati dagba ọja kan, eyiti o ṣajọpọ ọja naa siwaju.Ifihan agbara Fuluorisenti ti wa ni ikojọpọ nigbagbogbo pẹlu dida ọja naa, nitorinaa riri wiwa ti acid nucleic afojusun.

ikanni

FAM Coxsackie kokoro iru A16
ROX ti abẹnu Iṣakoso

Imọ paramita

Ibi ipamọ

≤-18℃

Selifu-aye

12 osu

Apeere Iru

Titun gba ọfun swabs

CV

≤10.0%

Ct

≤38

LoD

2000 Awọn ẹda/ml

Awọn ohun elo ti o wulo

Applied Biosystems 7500 Real-Time PCR SystemsSLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR eto

Eto Wiwa Iwọn otutu Ibakan ti Fluorescence gidi-gidi Rọrun Amp HWTS1600

Sisan iṣẹ

Aṣayan 1

Niyanju isediwon reagent: Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) ati Makiro & Micro-Idanwo Aifọwọyi Nucleic Acid Extractor (HWTS-3006)

Aṣayan 2

Niyanju isediwon reagent: Makiro & Micro-Test Ayẹwo Tu Reagent (HWTS-3005-8).


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