4 Iru Awọn ọlọjẹ Nucleic Acid
Orukọ ọja
HWTS-RT075-Didi-sigbe 4 Awọn oriṣi ti Awọn ọlọjẹ atẹgun Apoti Acid Nucleic Acid (Fluorescence PCR)
Arun-arun
Arun Iwoye Corona 2019, tọka si bi “COVID-19”, tọka si ẹdọforo ti o fa nipasẹ ikolu SARS-CoV-2.SARS-CoV-2 jẹ coronavirus ti o jẹ ti iwin β.COVID-19 jẹ arun ajakalẹ-arun ti atẹgun nla, ati pe olugbe ni ifaragba gbogbogbo.Ni lọwọlọwọ, orisun ti akoran jẹ pataki awọn alaisan ti o ni akoran nipasẹ SARS-CoV-2, ati pe awọn eniyan ti o ni akoran asymptomatic le tun di orisun ti akoran.Da lori iwadii ajakale-arun lọwọlọwọ, akoko isubu jẹ awọn ọjọ 1-14, pupọ julọ awọn ọjọ 3-7.Iba, Ikọaláìdúró gbigbẹ ati rirẹ jẹ awọn ifarahan akọkọ.Awọn alaisan diẹ ni awọn aami aiṣan bii isunmi imu, imu imu, ọfun ọfun, myalgia ati gbuuru, ati bẹbẹ lọ.
Aarun ajakalẹ-arun, ti a mọ ni “aisan”, jẹ arun ajakalẹ-arun atẹgun ti o fa nipasẹ ọlọjẹ aarun ayọkẹlẹ.O ti wa ni gíga àkóràn.O ti wa ni o kun tan nipasẹ iwúkọẹjẹ ati sneezing.O maa n jade ni orisun omi ati igba otutu.Awọn ọlọjẹ aarun ayọkẹlẹ ti pin si aarun ayọkẹlẹ A (IFV A), aarun ayọkẹlẹ B (IFV B), ati aarun ayọkẹlẹ C (IFV C) awọn oriṣi mẹta, gbogbo wọn jẹ ti ọlọjẹ alalepo, ti o fa arun eniyan paapaa fun awọn ọlọjẹ A ati B, o jẹ ẹyọkan. -stranded, segmented RNA kokoro.Kokoro aarun ayọkẹlẹ jẹ akoran ti atẹgun nla, pẹlu H1N1, H3N2 ati awọn ẹya-ara miiran, eyiti o ni itara si iyipada ati ibesile agbaye."Iyipada" ntokasi si iyipada ti aarun ayọkẹlẹ A kokoro, Abajade ni ifarahan ti kokoro tuntun "subtype".Awọn ọlọjẹ aarun ayọkẹlẹ B ti pin si awọn idile meji, Yamagata ati Victoria.Kokoro aarun ayọkẹlẹ B nikan ni fiseete antigenic, ati pe o yago fun eto iwo-kakiri ati imukuro eto ajẹsara eniyan nipasẹ iyipada rẹ.Sibẹsibẹ, iyara itankalẹ ti ọlọjẹ aarun ayọkẹlẹ B jẹ o lọra ju ti ọlọjẹ aarun ayọkẹlẹ eniyan A.Kokoro aarun ayọkẹlẹ B tun le fa awọn akoran atẹgun eniyan ati ja si awọn ajakale-arun.
Kokoro syncytial ti atẹgun (RSV) jẹ ọlọjẹ RNA kan, ti o jẹ ti idile paramyxoviridae.O ti gbejade nipasẹ awọn isunmi afẹfẹ ati isunmọ isunmọ ati pe o jẹ pathogen akọkọ ti ikolu ti atẹgun atẹgun ninu awọn ọmọde.Awọn ọmọde ti o ni RSV le ni idagbasoke bronchiolitis ti o lagbara ati pneumonia, eyiti o ni ibatan si ikọ-fèé ninu awọn ọmọde.Awọn ọmọ ikoko ni awọn aami aiṣan ti o lagbara, pẹlu iba giga, rhinitis, pharyngitis ati laryngitis, ati lẹhinna bronchiolitis ati pneumonia.Diẹ ninu awọn ọmọde aisan le ni idiju pẹlu otitis media, pleurisy ati myocarditis, bbl
ikanni
FAM | SARS-CoV-2 |
VIC(HEX) | RSV |
CY5 | IFV A |
ROX | IFV B |
NED | Iṣakoso ti abẹnu |
Imọ paramita
Ibi ipamọ | 2-8°C |
Selifu-aye | 12 osu |
Apeere Iru | Oropharyngeal swab |
Ct | ≤38 |
LoD | SARS-CoV-2: 150Ẹda/ml Kokoro aarun ayọkẹlẹ A / Kokoro aarun ayọkẹlẹ B / Kokoro syncytial ti atẹgun: 300Awọn ẹda/mL |
Ni pato | Ko si ifaseyin agbelebu pẹlu coronavirus eniyan SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, ọlọjẹ parainfluenza iru 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus eniyan, enterovirus A, B, C, D, ọlọjẹ ẹdọforo eniyan, ọlọjẹ epstein-barr, ọlọjẹ measles, ọlọjẹ cytomegalo eniyan, rotavirus, norovirus, ọlọjẹ parotitis, virus varicella-zoster, legionella, bordetella pertussis, haemophilus influenzae. staphylococcus aureus, streptococcus pneumoniae, s.pyogenes, klebsiella pneumoniae, mycobacterium iko, ẹfin aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci ati cryptococcus ọmọ ikoko ati eda eniyan jenomic nucleic acid. |
Awọn ohun elo ti o wulo | Ohun elo Biosystems 7500 Real-akoko PCR System, Applied Biosystems 7500 Yara gidi-akoko PCR Systems QuantStudio®5 Real-akoko PCR Systems |
Sisan iṣẹ
Aṣayan 1.
Niyanju isediwon reagent: Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) ati Makiro & Micro-Igbeyewo Aifọwọyi Nucleic Acid Extractor (HWTS-3006).
Aṣayan 2.
Aṣeyọri isediwon ti a ṣe iṣeduro: Iyọkuro Acid Nucleic tabi Reagent Mimọ (YDP302) nipasẹ Tiangen Biotech(Beijing) Co., Ltd.