15 Awọn oriṣi ti Ewu Ewu Eniyan Papillomavirus E6/E7 Gene mRNA
Orukọ ọja
HWTS-CC005A-15 Awọn oriṣi ti Ewu to gaju Papillomavirus E6/E7 Gene mRNA Apo Iwari (Fluorescence PCR)
Arun-arun
Akàn jẹ ọkan ninu awọn oriṣi ti o wọpọ julọ ti akàn obinrin ni agbaye, ati pe iṣẹlẹ rẹ ni ibatan pẹkipẹki pẹlu papillomavirus eniyan (HPV), ṣugbọn ipin diẹ ti awọn akoran HPV le dagba sinu akàn.HPV ti o ni ewu ti o ga julọ n ṣe akoran awọn sẹẹli epithelial cervical ati ṣe agbejade awọn oncoprotein meji, E6 ati E7.Amuaradagba yii le ni ipa lori ọpọlọpọ awọn ọlọjẹ cellular (gẹgẹbi awọn ọlọjẹ suppressor pRB ati p53), fa gigun sẹẹli naa, ni ipa lori iṣelọpọ DNA ati iduroṣinṣin genome, ati dabaru pẹlu awọn idahun antiviral ati antitumor.
ikanni
| ikanni | Ẹya ara ẹrọ | Genotype ni idanwo |
| FAM | Idaduro Idahun HPV 1 | HPV16, 31, 33, 35, 51, 52, 58 |
| VIC/HEX | Jiini β-actin eniyan | |
| FAM | Idaduro Idahun HPV 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Jiini INS eniyan |
Imọ paramita
| Ibi ipamọ | Omi: ≤-18℃ |
| Selifu-aye | osu 9 |
| Apeere Iru | sẹẹli exfoliated cervical |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 idaako/ml |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Niyanju isediwon reagent: Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Isediwon yẹ ki o waiye muna ni ibamu si awọn ilana fun lilo .Iwọn didun elution ti a ṣe iṣeduro jẹ 50μL.Ti ayẹwo ko ba jẹ digested patapata, da pada si igbesẹ 4 fun atunkọ.Ati lẹhinna ṣe idanwo ni ibamu si awọn ilana fun lilo.
Niyanju isediwon reagent: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431).Isediwon yẹ ki o waiye ni ibamu si awọn itọnisọna fun lilo ni muna (Ni igbesẹ 5, ilọpo meji ifọkansi ti ojutu ṣiṣẹ DNaseI, iyẹn ni, mu 20μL ti RNase-Free DNaseI (1500U) ojutu ọja sinu tube tuntun RNase-Free centrifuge, ṣafikun 60μL ti ifipamọ RDD, ati dapọ rọra).Iwọn didun elution ti a ṣe iṣeduro jẹ 60μL.Ti ayẹwo ko ba jẹ digested patapata, da pada si igbesẹ 5 fun atunkọ.Ati lẹhinna ṣe idanwo ni ibamu si awọn ilana fun lilo.
